Effect of PEA-LUT combination therapy on nitrotyrosine immunostaining and MDA levels. A marked increase in nitrotyrosine (A, see particularly A1 and C), staining was evident in the paw 35 days after initiation of CIA. A marked reduction was seen in the immunostaining for nitrotyrosine (B, see particularly B1 and C) in the paws of CIA-PEA-LUT mice. Densitometry analysis of immunocytochemistry photographs (n = 5) for nitrotyrosine from paw sections was assessed (C). The assay was carried out by using Optilab Graftek software on a Macintosh personal computer (CPU G3-266). Data are expressed as percentage of total tissue area. *P < 0.01 versus Sham-control. °P < 0.01 versus CIA. In addition, MDA levels, a marker of lipid peroxidation, were evaluated. A substantial increase in MDA levels (D) was found in CIA-control mice 35 days after CII immunization. CIA-PEA-treated mice demonstrated a significant reduction in MDA levels (D). The combination therapy with PEA-LUT enhanced the reduction in MDA levels (D). Values are given as mean ± SEM of 20 animals for each group. *P < 0.01 versus Sham-control. °P < 0.01 versus CIA-control. #
P < 0.01 versus CIA-PEA. §
P < 0.01 versus CIA-LUT.