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Table 1 Sequences of the human gene-specific primers used for qPCR

From: NFAT3 and TGF-β/SMAD3 regulate the expression of miR-140 in osteoarthritis

Gene Sequence
(GenBank accession number)
FAK 5’-AGAAGTATGAGCTTGCTCAC (S)
(NM_153831.2) 5’-TGATCGCCGTATTTCTAGAC (AS)
GAPDH 5’-CAGAACATCATCCCTGCCTCT (S)
(NM_002046.3) 5’-GCTTGACAAAGTGGTCGTTGAG (AS)
IGFBP5 5’-TGAAGCAGTGAAGAAGGAC (S)
(NM_000599) 5’-CTGCTTTCTCTTGTAGAATC (AS)
MAZ 5’-GATCACCTCAACAGTCACGTC (S)
(NM_002383.2) 5’-CTGTGCACCTTCATGTGGTC (AS)
NFAT1 (NFATc2) 5’-AGAATCCATCCTGCTGGTTC (S)
(NM_012340) 5’-TCCATGTAGCCATGGAGCTG (AS)
NFAT2 (NFATc1) 5’-TCATTGACTGTGCCGGAATC (S)
(NM_172390) 5’-AAGTTGTGGCCAGACAGGAC (AS)
NFAT3 (NFATc4) 5’-AGAACTGGACTCAGAGGATG (S)
(NM_004554) 5’-ATGGAGGTGATGCGGATG (AS)
NFAT4 (NFATc3) 5’-CCAGCCCGGGAGACTTCAATAGAT (S)
(NM_173165) 5’-GCCCAGGAGCTTCACAACAGGAT (AS)
NFAT5 (TonEBP) 5’-CTGAGCAGAGCTGCAGTATG (S)
(NM_138713) 5’-GTTGTCCGTGGTAAGCTGAG (AS)
NMP4 5’-CGTACTTCTGGCCTTCTATC (S)
(AB070238) 5’-CAGTCATCAGTCCTGTAGAC (AS)
SMAD3 5’-GTCTGCAAGATCCCACCAGG (S)
(NM_005902) 5’-CTTGTCAAGCCACTGCAAAG (AS)
SREBF2 5’-TGAGATCCATCTGACTGCTG (S)
(NM_004599) 5’-CCTCTGGGCACAGTATAGAC (AS)
WWP2 5’-CAAGGTGCATAATCGTCAAC (S)
(NC_000016) (variants 1, 3) 5’-GATGCGGTGCCTAGCAGTTC (AS)
WWP2 5’-TCCTCCTGTCTCATGAGGTG (S)
(NC_000016) (variants 1, 2) 5’-GCCTATAAAGCGAAAGTAGG (AS)
  1. S sense, AS antisense.