Table 1 Sequences of the human gene-specific primers used for qPCR
From: NFAT3 and TGF-β/SMAD3 regulate the expression of miR-140 in osteoarthritis
Gene | Sequence |
|---|---|
(GenBank accession number) | |
FAK | 5’-AGAAGTATGAGCTTGCTCAC (S) |
(NM_153831.2) | 5’-TGATCGCCGTATTTCTAGAC (AS) |
GAPDH | 5’-CAGAACATCATCCCTGCCTCT (S) |
(NM_002046.3) | 5’-GCTTGACAAAGTGGTCGTTGAG (AS) |
IGFBP5 | 5’-TGAAGCAGTGAAGAAGGAC (S) |
(NM_000599) | 5’-CTGCTTTCTCTTGTAGAATC (AS) |
MAZ | 5’-GATCACCTCAACAGTCACGTC (S) |
(NM_002383.2) | 5’-CTGTGCACCTTCATGTGGTC (AS) |
NFAT1 (NFATc2) | 5’-AGAATCCATCCTGCTGGTTC (S) |
(NM_012340) | 5’-TCCATGTAGCCATGGAGCTG (AS) |
NFAT2 (NFATc1) | 5’-TCATTGACTGTGCCGGAATC (S) |
(NM_172390) | 5’-AAGTTGTGGCCAGACAGGAC (AS) |
NFAT3 (NFATc4) | 5’-AGAACTGGACTCAGAGGATG (S) |
(NM_004554) | 5’-ATGGAGGTGATGCGGATG (AS) |
NFAT4 (NFATc3) | 5’-CCAGCCCGGGAGACTTCAATAGAT (S) |
(NM_173165) | 5’-GCCCAGGAGCTTCACAACAGGAT (AS) |
NFAT5 (TonEBP) | 5’-CTGAGCAGAGCTGCAGTATG (S) |
(NM_138713) | 5’-GTTGTCCGTGGTAAGCTGAG (AS) |
NMP4 | 5’-CGTACTTCTGGCCTTCTATC (S) |
(AB070238) | 5’-CAGTCATCAGTCCTGTAGAC (AS) |
SMAD3 | 5’-GTCTGCAAGATCCCACCAGG (S) |
(NM_005902) | 5’-CTTGTCAAGCCACTGCAAAG (AS) |
SREBF2 | 5’-TGAGATCCATCTGACTGCTG (S) |
(NM_004599) | 5’-CCTCTGGGCACAGTATAGAC (AS) |
WWP2 | 5’-CAAGGTGCATAATCGTCAAC (S) |
(NC_000016) (variants 1, 3) | 5’-GATGCGGTGCCTAGCAGTTC (AS) |
WWP2 | 5’-TCCTCCTGTCTCATGAGGTG (S) |
(NC_000016) (variants 1, 2) | 5’-GCCTATAAAGCGAAAGTAGG (AS) |