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Figure 1 | Arthritis Research & Therapy

Figure 1

From: Deficient spontaneous in vitro apoptosis and increased tmTNF reverse signaling-induced apoptosis of monocytes predict suboptimal therapeutic response of rheumatoid arthritis to TNF inhibition

Figure 1

TNFR2:Ig triggered reverse signaling via transmembrane (tm)TNF induces apoptosis in monocytes from rheumatoid arthritis (RA) patients but not from healthy controls. (A) Monocytes from the peripheral blood of healthy donors (HD) and from RA patients (RA) were cultured with IgG or TNFR2:Ig for 16 hours. Box plot depicts the rate of spontaneous apoptosis in vitro (SIA) cultures with IgG (dark gray bars) and of tmTNF reverse signaling-induced apoptosis (tmTNF RSA) in the presence of TNFR2:Ig (light gray bars) in healthy controls (HD) (n = 10) and RA patients (RA) (n = 13). Significant differences are as indicated. (B) Representative dot plots of annexin V- and propidium iodide-stained monocytes from a healthy control (left panels) and from one RA patient (right panels) after incubation with IgG or TNFR2:Ig for 16 hours. Upper panels show spontaneous apoptosis in vitro (SIA) (IgG), lower panels tmTNF reverse signaling induced apoptosis (tmTNF RSA) (TNFR2:Ig).

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Arthritis Research & Therapy

ISSN: 1478-6362

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