Figure 3

Subcellular localization and function of protein tyrosine phosphatase, non-receptor type 22 (PTPN22) isoforms. (A) 293 T cells were transfected with 1 μg of plasmid vectors expressing indicated FLAG-tagged PTPN22 isoforms. Cytoplasmic and nuclear extract was separately prepared from the transfected cells and subjected to Western blotting with indicated antibodies. The protein product of each isoform is marked with ‘*’. (B) Jurkat cells were transfected with a NFAT-luc reporter, 10 ug of pCMV2B expressing indicated FLAG-PTPN22 isoforms, and a TK-Renilla reporter. The transfected cells were then stimulated with anti-CD3 overnight. A fraction of the transfected cells was subjected to Western blotting with anti-FLAG (the top panel). The luciferase activity of the remaining cells was analyzed. Normalized firefly luciferase activity was calculated as described in Methods and is shown in the bottom panel. In each experiment, the normalized value from cells transfected with the empty expression vector was arbitrarily set as 100. The data shown are the means and standard deviations of three independent experiments. Statistical significance was calculated with one-way ANOVA followed by Dunn’s test comparing non-full-length isoforms to PTPN22.1. *P = 0.0158.