Figure 5

Potentiation of IL-1β-stimulated NGF expression and release by visfatin/NAMPT. Primary cultures of mouse articular chondrocytes were stimulated or not for 24 hours either with IL-1β (1 ng/mL) or visfatin/NAMPT (5 μg/mL) or with IL-1β (1 ng/mL) and visfatin/NAMPT (5 μg/mL) (n = 3). The mRNA expression of NGF (A) and the NGF protein levels in chondrocyte conditioned media were determined (B). *P <0.05, ***P <0.001. IL-1β, interleukin 1β; NAMPT, nicotinamide phosphoribosyltransferase activity. Supress activity; NGF, nerve growth factor.