V region subfamily
|
FR negative
|
CDR negative
|
CDR positive
|
---|
VH1-3
|
P ≤ 0.002b
|
P ≤ 0.03b
| |
VH1-46
| | |
P < 0.02
|
VH1-69
|
P ≤ 0.01(2),eP ≤ 0.02 (2),fP < -0.05
| |
P < 0.01
|
VH3-23
|
P ≤ 0.02c
|
P ≤ 0.01c
| |
VH3-30
|
P ≤ 0.001
| | |
VH3-74
|
P ≤ 0.02
| | |
VH4-39
| | |
P < 0.02
|
VH4-4
|
P ≤ 0.01d
|
P ≤ 0.03d
| |
Vκ1-39
| | |
P < 0.03
|
Vκ2-28
| | |
P < 0.04
|
- aThe focused binomial test[18] was used to estimate selection, yielding P values with statistical significance at <0.05.
- b,c,dThese three VH genes showed negative selection in both FR and CDR.
- e,fTwo and two individual VH genes each, respectively, displayed identical P values.