Plasma cells within granulomatous inflammation display signs pointing to autoreactivity and destruction in granulomatosis with polyangiitis

Table 1 Overview of selection ( P values) against or for replacement mutations within framework region (FR) and CDR of plasma cell-derived Ig genes in nasal tissue in GPA ^a

V region subfamily	FR negative	CDR negative	CDR positive
VH1-3	P ≤ 0.002^b	P ≤ 0.03^b
VH1-46			P < 0.02
VH1-69	P ≤ 0.01(2),^eP ≤ 0.02 (2),^fP < -0.05		P < 0.01
VH3-23	P ≤ 0.02^c	P ≤ 0.01^c
VH3-30	P ≤ 0.001
VH3-74	P ≤ 0.02
VH4-39			P < 0.02
VH4-4	P ≤ 0.01^d	P ≤ 0.03^d
Vκ1-39			P < 0.03
Vκ2-28			P < 0.04

^aThe focused binomial test[18] was used to estimate selection, yielding P values with statistical significance at <0.05.
^b,c,dThese three VH genes showed negative selection in both FR and CDR.
^e,fTwo and two individual VH genes each, respectively, displayed identical P values.