Acute oral administration of docosahexaenoic acid restores horizontal exploratory activity and reduces knee edema. At day 15, 30 mg/kg docosahexaenoic acid (DHA) was given orally (p.o.) to mice that received injections of Complete Freund’s Adjuvant (CFA). Behavioral analysis was performed at 1, 2 and 24 hours after DHA administration. Knee edema was measured at hours 2 and 24 after DHA administration. Acute oral administration of DHA did not modify either spontaneous pain (A) or vertical rearing (C) in CFA-injected mice. (B) Horizontal exploratory activity was increased after DHA administration as compared to CFA-injected mice treated with vehicle. (D) Knee edema was decreased at 2 and 24 hours after DHA administration. Each bar represents the mean ± SEM. *P < 0.05 vs. intraarticular (i.a.) CFA + p.o. vehicle according to respective times by two-way repeated-measures analysis of variance followed by post hoc Bonferroni correction.