Table 2 Quantitative PCR primer list a
From: Novel immortal human cell lines reveal subpopulations in the nucleus pulposus
Symbol | Gene name | Tissue | Forward primer | Reverse primer |
|---|---|---|---|---|
ACAN | Aggrecan | AC, NP | GCAGCTGGGCGTTGTCA | TGAGTACAGGAGGCTTGAGGACT |
CA12 | Carbonic anhydrase XII | NP | ATCCAACTAATGCCACCACCAA | TGAGACCACGAAGAGACTGGCT |
COL1A1 | Collagen type I, collagen α1 | AF | TGGAGAGTACTGGATTGACCCC | TGCAGAAGACTTTGATGGCATC |
COL2A1 | Collagen type II, collagen α1 | AC, NP | TGGGTGTTCTATTTATTTATTGTCTTCCT | GCGTTGGACTCACACCAGTTAGT |
COMP | Cartilage oligomeric matrix protein | AC | CAAGGCCAACAAGCAGGTTTG | CAGTTATGTTGCCCGGTCTCA |
CD24 | Cluster of differentiation 24 | NP | CCACGCAGATTTATTCCAGTGA | GCCAACCCAGAGTTGGAAGTAC |
FOXF1 | Forkhead box F1 | NP | CCCACACAGGAATTCTGCTGA | TTCCCCCACTTCTGCCATT |
KRT19 | Keratin 19 | NC, NP | GCAGTCACAGCTGAGCATGAA | TCCGTTTCTGCCAGTGTGTCT |
PAX1 | Paired box 1 | NP | AGAGCCTGACATCGCCTGTTAA | CGCTTTCCTTTATTCAGAGGCA |
PTN | Pleiotrophin | NP | AGAAGCAATTTGGCGCGGA | TTCAGGTCACATTCTCCCCAGG |
SOX9 | SRY-box 9 | AC, NP | AGTACCCGCACCTGCACAAC | CGCTTCTCGCTCTCGTTCAG |
T | Brachyury T | NC | CCACCTGCAAATCCTCATCCT | TTGGAGAATTGTTCCGATGAGC |