Figure 3From: Naproxen affects osteogenesis of human mesenchymal stem cells via regulation of Indian hedgehog signaling moleculesEffect of naproxen (Npx) on mineral deposition by mesenchymal stem cells (MSCs) cultured in osteogenic differentiation medium. Alizarin red S staining of mineral deposition in the extracellular matrix after MSCs were cultured in osteogenic differentiation medium without (control) (A) or with 100 μg/mL Npx (B) for 21 days (bar represents 50 μm); (C) the absorbance value of solubilized alizarin red S measured at 570 nm. The results are shown as mean ± SD of three independent experiments with MSCs isolated from three different donors. P <0.05 was determined as statistically significant.Back to article page