- Meeting abstract
- Open Access
Digital Image Analysis to Quantify Inflammation in Synovial Tissue
Arthritis Research & Therapyvolume 1, Article number: S34 (1999)
Computer-based assessment of images acquired in a microscope requires an attached video camera transferring the images to a computer and a screen. The analytical decisions made by the computer equipped with a special software program will be displayed on the screen in discriminating pseudocolors, enabling the operator to monitor and accept or discard the results of a given field. Quantification of cells expressing cell surface antigens or intracellular antigens can be readily done in immunohistochemically stained tissue sections from synovial tissue. Conventional microscopy is a good tool for counting but poor at measuring areas, staining intensity, or distances, which can be handled in a superior way by computer-assisted analysis. We have successfully applied this technology to quantify the incidence of various cell types, intimal layer thickness, and cytokine-producing cells in rheumatoid synovial tissue specimens. Computerized image analysis offers a major advantage in sequential studies to evaluate changes in response to therapy.
Bjork L, Fehniger TE, Andersson J, Andersson U: Computerized assessment of multiple human cytokines at the single cell level using image analysis. J Leukocyte Biol. 1996, 59: 287-295.
Litton M, Dohlsten M, Hansson J, et al: Tumor therapy with an antibody-targeted superantigen generates a dichotomy between local and systemic immune responses. Am J Pathol. 1997, 150: 1607-1618.
Andersson J, Fehniger TE, Patterson BK, et al: Early reduction of immune activation in lymphoid tissue following highly active HIV therapy. AIDS. 1998, 12: 123-129. 10.1097/00002030-199811000-00004.
Cunnane G, Bjork L, Ulfgren A-K, FitzGerald O, Bresnihan B, Andersson U: Quantitative analysis of synovial membrane inflammation: a comparison between automated and conventional microscopic measurements. Ann Rheum Dis. 1999, 58: 493-499.
Litton M, Dohlsten M, Rosendahl A, et al: The distinct role of CD4+ and CD8+ T cells during the anti-tumor effects of targeted super-antigens. Br J Cancer. 1999, 81: 359-66. 10.1038/sj.bjc.6690701.
Andersson U, Litton M, Fehniger TE, Ulfgren AK, Andersson J: Detection and quantification of cytokine-producing cells by immunostaining. In Detection and quantification of gene expression. Edited by Patterson BK. New York: Springer–Verlag; . 1999