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  • Meeting abstract
  • Open Access

Comparison of different immunoassays detecting anti-chromatin autoantibodies in systemic lupus erythematosus (SLE)

  • 1,
  • 1 and
  • 1
Arthritis Research & Therapy20024 (Suppl 1) :70

https://doi.org/10.1186/ar515

  • Received: 15 January 2002
  • Published:

Keywords

  • Public Health
  • Systemic Lupus Erythematosus
  • Retrospective Study
  • Autoimmune Disease
  • Systemic Lupus Erythematosus Patient

Introduction

Anti-double-strand DNA (dsDNA) autoantibodies are considered a hallmark of SLE and many assays have been developed to improve the determination of these antibodies. Recently, autoantibodies to nucleosome, the fundamental unit of chromatin, have been shown to constitute a new and very specific marker of SLE.

Methods

To evaluate the diagnostic value of (A) Crithidia lucillae indirect immunofluorescence (CLIFT), anti-dsDNA immunoassays using (B) purified dsDNA (Quanta LiteTM dsDNA ELISA INOVA, San Diego, CA), (C) recombinant plasmid dsDNA (EliATM, Pharmacia, Freiburg, Germany) and (D) anti-nucleosome immunoassay (Quanta Lite Chromatin ELISATM, INOVA, San Diego, CA), we investigated in a prospective study all the sera sent to our laboratory for anti-dsDNA detection during 2 months. 122 sera were enrolled in this study of which 16 appear to be from SLE patients and we assessed the sensitivity and specificity of the assays. We also compared a group of 30 SLE patients with other autoimmune diseases (19 cutaneous lupus, 22 Sjögren's syndrome, 14 systemic scleroderma) in a retrospective study.

Results

In the prospective study, the sensitivity for SLE was 13%, 53%, 47% and 60% for test A, B, C and D, respectively; the specificity was 99%, 94%, 94% and 99% for test A, B, C and D, respectively. Results were similar in the retrospective study.

Conclusion

Anti-nucleosome autoantibodies are sensitive and specific markers for SLE. However, other studies are necessary to evaluate if they are also useful in monitoring disease activity.

Authors’ Affiliations

(1)
Division of Immunology and Allergy, Geneva, Switzerland

Copyright

© BioMed Central Ltd 2002

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