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Table 2 Effects of CD40 ligation and LPS on IL-10 and tumor necrosis factor alpha (TNF-α) production by synovial fluid (SF) macrophages and normal peripheral blood (PB) monocytesa

From: Regulation of CD154-induced interleukin-12 production in synovial fluid macrophages

  

SF macrophages

PB monocytes

Cytokine

Incubation period (h)

Medium

J558L

CD154

LPS

Medium

J558L

CD154

LPS

IL-10

24

305 ± 193

161 ± 101

566 ± 208*

1,973 ± 788*

90 ± 49

99 ± 51

705 ± 465*

2,803 ± 938*

 

72

15 ± 11b

33 ± 19c

174 ± 55b,*

734 ± 357b

10 ± 4

18 ± 7

734 ± 414*

1,389 ± 752*

TNF-α

24

46 ± 39

20 ± 13

270 ± 62*

1,397 ± 437*

58 ± 35

44 ± 29

111 ± 40

3,410 ± 1,541*

 

72

<1

<1c

627 ± 220*,†

623 ± 344*

23 ± 14

19 ± 18

47 ± 19

242 ± 133*

  1. aSF macrophages and PB monocytes were cultured with irradiated control J558L-cells (1:5), CD154 tranfectants (1:5), or LPS (1 μg/ml) for 24 or 72 hours. Cytokine concentrations were determined using cytokine specific ELISAs. Results are presented as mean ± SEM pg/ml. n = 6 except bn = 5 and cn = 4. *P < 0.05 when compared with cells cultured with transfectant controls or in medium only at the respective time point. P < 0.05 when compared with PB monocytes cultured in the presence of TNF-α for 72 hours. LPS, lipopolysaccharide.