Figure 5
Cytometric analysis of secreted cytokines. (a) Cells can be analyzed after short-term activation ex vivo (5–10 h). (b) A bispecific cytokine affinity matrix, specific for a cytokine of choice and a cell-surface molecule, is attached to the viable cells. During a short culture period (15-30 min), secreted cytokines are bound and then stained with a second cytokine-specific antibody.