Figure 4
From: DEK binding to class II MHC Y-box sequences is gene- and allele-specific
DEK and NF-Y participation in the DQA1*0101 Y-box binding complex demonstrated by 'supershift' assay. Namalwa nuclear extract (5 μg) was preincubated with the following antibody reagents before addition of radiolabeled probe containing DQA1*0101 Y-box sequence: lane 2, no antibody reagent; lane 3, 2 μl normal human serum; lane 4, 1 μl high-titer anti-DEK human antiserum; lane 5, 1 μl anti-NF-YA (subunit) monoclonal antibody; lane 6, 1 μl of anti-DEK plus 1 μl of anti-NF-YA monoclonal antibody. Lane 1 contains probe without nuclear extract or antibody reagent.