Figure 1From: Extracellular mitochondrial DNA and oxidatively damaged DNA in synovial fluid of patients with rheumatoid arthritisPolymerase chain reaction (PCR) amplification of mtDNA from plasma and synovial fluid (SF) samples of patients with rheumatoid arthritis (RA). SDS–polyacrylamide-gel electrophoresis of PCR-amplified mitochondrial DNA (mtDNA) fragments (453 bp) of plasma and SF of five patients with RA. A semi-quantitative method was used to assign the PCR product intensities to positive or negative categories. An arbitrary cutoff point was assigned that was relative to the intensity of the 676-bp band (arrow) of the molecular mass marker on the gel. Lane 1, molecular weight marker; lane 2, water only (control); lane 3, plasma from patient 1 (plasma-1); lane 4, SF from patient 1 (SF-1); lane 5, plasma-2; lane 6, SF-2; lane 7, plasma-3; lane 8, SF-3; lane 9, plasma-4; lane 10, SF-4. The samples in lanes 11 (plasma-5) and 12 (SF-5) were spiked with 10 ng of purified mtDNA.Back to article page