Table 2 Antigens, mitogens and IL-2
Antigen | Working concentration | Source |
|---|---|---|
GroEL (E. coli HSP60) | 10 μg/ml | Bioquote Ltd, York, UK |
Human heat shock protein | 10 μg/ml | Bioquote Ltd |
Yersinia entercolitica lysate | 570 μg/ml | Microbiology Department, Queen Elizabeth Hospital, Birmingham, UK |
Salmonella typhimurium lysate | 300 μg/ml | Microbiology Department, Queen Elizabeth Hospital |
Streptolysin O (SLO) | 10 μg/ml | Sigma Chemical Co., Poole, UK |
Tetanus Toxoid (TT) | 10 μg/ml | Statens Serum Institut, Copenhagen, Denmark |
IL-2 | 50 U/ml | EuroCetus BV, Amsterdam, Holland |
Phytohaemagglutinin HA15 (PHA) | 1 μg/ml | Sigma Chemical Co. |