Differential gene expression in pristane-induced arthritis susceptible DA versus resistant E3 rats

Table 3 Genes differentially expressed in naïve (day 0) DA versus E3 rats

Accession no.	Gene ID	Significance	Affymetrix	Custom-made	FACS	ELISA
Upregulated
X56596	MHC class II antigen RT1.B-1 β-chain (MhcII)	T	I (56.8)	NC	I (1.2); P < 0.08	ND
X00923	IgE heavy chain (Ig ε)	S	I (3.6)	I (1.9); P < 0.1	ND	I (1.4); P < 0.05
D49955	Bone marrow stromal cell antigen 1 (Bst1)	T	I (3.7)	I (2.0); P < 0.1	ND	ND
Downregulated
S81289	IgM κ chain variable region (CDR1 to CDR3 region; Ig κ)	S	D (-3.6)	D (-1.6); P < 0.0005	NC	ND

To be regarded as differentially expressed a gene must be differentially expressed in two biological samples analyzed by a minimum of two independent methods. Differential expression values in bold text are statistically significant by that particular method. Genes significantly differentially expressed by a minimum of two independent methods are denoted 'S'. Another subset of genes showed a strong tendency toward differential expression; these are denoted 'T'. ELISA, enzyme linked immunosorbent assay; FACS, fluorescence activated cell sorting; NC, no change (for these genes, no statistically significant differential expression could be shown); ND, not determined.

ISSN: 1478-6362