| | TNF-α |
|---|
Treatment | Control | +VIP |
Control | 1400 ± 62.02 | 630.3 ± 61.55 (55%) |
Anti-IL-10 | 3041 ± 624.9 | 2195 ± 224.9 (28%) |
Exogenous IL-10 | 119.5 ± 6.944 | 88.26 ± 14.48 (26%) |
- Macrophage-colony stimulating factor (M-CSF)-primed monocyte-derived macrophages, plated at a density of 1×105 cells/well, were stimulated by 1 ng/ml LPS in the presence or absence of 10-6M VIP. IL-10 modulation of VIP regulation of TNF-α production was assessed by addition of 10 μg/ml neutralising anti-IL-10 (9D7) or 10 ng/ml IL-10. Results with an isotype-matched control antibody did not differ significantly from the control sample presented in this table. The resulting cultures were incubated for 24 hours at 37°C in a 5% CO2 humidified atmosphere, after which time supernatants were harvested and assayed for TNF-α by ELISA. Data are mean TNF-α levels in pg/ml and percentage suppression by VIP in parentheses of triplicate culture supernatants ± SD, showing a representative of n = 3 experiments. LPS, lipopolysaccharide; TNF-α, tumour necrosis factorα; VIP, vasoactive intestinal peptide.
