Laser speckle contrast analysis of the inflamed mouse knee joint. (A) Time course of the effect of locally administered URB597 (closed circles) vs. vehicle (open circles) on vascular perfusion. There was a significant reduction in local blood flow after URB597 administration, when compared to vehicle. P <0.0001; two-way ANOVA; n =6 to 7. (B) Dose-response profile for the effects of URB597 20 mins after administration. Local blood flow was significantly reduced by only low-dose URB597 (3.0 mg/kg). **P <0.01 one-way ANOVA with Dunnett's post hoc test vs. vehicle; n =5 to 7. (C) Both AM251 (0.2 mg/kg) and AM630 (0.2 mg/kg) blocked the effect of URB597 (3.0 mg/kg) on blood flow in the inflamed mouse knee joint. Data are means ± SEM.*P <0.05, one-way ANOVA with Bonferroni's post hoc test vs. URB597 3.0 mg/kg; n =6 to 8. ANOVA, analysis of variance; SEM, standard error of the mean.