Figure 5From: Tapping into the endocannabinoid system to ameliorate acute inflammatory flares and associated pain in mouse knee jointsHindlimb weight bearing in mice with acute experimental joint inflammation. (A) Time course of the effects of URB597 (3.0 mg/kg) on inflamed hindlimb weight bearing (baseline (BL) values represent pre-inflammation induction). Over the course of 3 hr, URB597 significantly improved hindlimb weight bearing when compared to vehicle. P <0.0001; two-way ANOVA; n =12. (B) Hindlimb incapacitance 30 minutes after local administration of URB597 to acutely inflamed knees. The anti-nociceptive effect of URB597 (3.0 mg/kg) was blocked by the CB1 receptor antagonist AM251 (0.2 mg/kg), but not the CB2 receptor antagonist AM630 (0.2 mg/kg). When URB597 was administered to the contralateral knee, there was no observable effect on hindlimb weight bearing. Data are means ± SEM. *P <0.05, **P <0.01; one-way ANOVA with Bonferroni's post hoc test; n = 9 to 14. ANOVA, analysis of variance; SEM, standard error of the mean.Back to article page