Figure 5

Effects of CCX8037 on CIA mice. (A-C) CCX8037 (3 mg/kg or 10 mg/kg) or vehicle (all n =12) was injected subcutaneously twice daily from day -7 to day 14. Disease severity was recorded as the clinical arthritis score until day 14 (A). Representative photographs showing hematoxylin and eosin staining of the ankle joints from mice with CIA treated with vehicle (B(i)) or CCX8037 (10 mg/kg) (B(ii)). Bar, 300μμm. Inflammatory cell infiltration in the right ankle joint was scored with a pathological score (B(iii)). Representative radiographs of the ankle joints of CIA mice treated with vehicle (C(i)) or CCX8037 (10 mg/kg) (C(ii)). Bone erosion in the bilateral ankle joints was scored with radiological score (C(iii)). Values are the mean ± SEM of each group. *P <0.05, CCX8037 (10 mg/kg) versus vehicle. (D-F) To investigate the therapeutic effects of CCX8037, at day 5 after the second immunization, mice were divided into two groups with equal average arthritis score. CCX8037 (10 mg/kg) or vehicle (all n =12) was injected subcutaneously twice daily from day 5 to day 18. Disease severity was recorded as the clinical arthritis score (D). Representative hematoxylin and eosin staining of the ankle joints of CIA mice treated with vehicle (E(i)) or CCX8037 (10 mg/kg) (E(ii)). Bar, 300μμm. Pathological score (E(iii)). Representative radiographs of the ankle joints of CIA mice treated with vehicle (F(i)) or CCX8037 (10 mg/kg) (F(ii)). Radiological score (F(iii)). Values are the mean ± SEM of each group. *P <0.05 versus vehicle. CCX8037, CC chemokine receptor (CCR) 9 antagonist; CIA, collagen-induced arthritis; SEM, standard error of the mean.