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Figure 2 | Arthritis Research & Therapy

Figure 2

From: Hypoxia and vitamin D differently contribute to leptin and dickkopf-related protein 2 production in human osteoarthritic subchondral bone osteoblasts

Figure 2

Osteocalcin, dickkopf-related protein 2 and leptin expression under hypoxic conditions. Human osteoarthritis osteoblasts were incubated for 24 hours (RT-PCR experiments) to determine the expression of osteocalcin (A), dickkopf-related protein 2 (DKK2) (B) and leptin (C) (n =7 to 10 experiments), or for 48 hours (enzyme-linked immunosorbent assay experiments) to measure leptin production (D) (n =8 experiments) under either normoxia or hypoxia (2% O2) in the presence of 50 nM 1,25-dihydroxyvitamin D3 (D3) or vehicle (0.1% ethanol; EtOH). *P <0.05. ND, Not detectable.

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