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Table 1 Oligonucleotides used for real-time RT-PCR a

From: Hypoxia and vitamin D differently contribute to leptin and dickkopf-related protein 2 production in human osteoarthritic subchondral bone osteoblasts

Gene   Sequence 5′-3′ Gene ID
RP29 S 5′-GGG TCA CCA GCA GCT CGA GA-3′ NM_001032.3
AS 5′-CAG ACA CGA CAA GAG CGA GA-3′
Leptin S 5′-GGC TTT GGC CCT ATC TTT TC-3′ NM_000230.2
AS 5′-GGA TAA GGT CAG GAT GGG GT-3′
DKK2 S 5′-GGG TTT TGC TGT GCT CGT-3′ NM_014421.2
AS 5′-TGG CTT TGG AGG AGT AGG TG-3′
Hif-1 S 5′-GAA AGC GCA AGT CCT CAA AG-3′ NM_181054.2
AS 5′-TGG GTA GGA CAT GGA GAT GC-3′
Hif-2 S 5′-TTG-ATG-TGG-AAA-CGG-ATG-AA-3′ NM_001430.4
AS 5′-GGA-ACC-TGC-TCT-TGC-TGT-TC-3′
Osteocalcin S 5′-CAT-GAG-AGC-CCT-CAC-A-3′ NM_199173.4
AS 5′-AGA-GCG-ACA-CCC-TAG-AC-3′
VEGFα S 5′-TGG-GCC-TTG-CTC-AGA-GCG-GA-3′ NM_001171629.1
AS 5′-GCC-TTG-CAA-CGC-GAG-TCTGT-3′
TGFβ1 S 5′-GCG-TGC-TAA-TGG-TGG-AAA-C-3′ NM_000660.5
AS 5′-GCT-GAG-GTA-TCG-CCA-GGA-A-3′
Sirt1 S 5′-GCT-GGA-ACA-GGT-TGC-GGG-AA-3′ NM_012238.4
AS 5′-GGG-CAC-CTA-GGA-CAT-CGA-GGA-3′
  1. S, sense; AS, Antisense; DKK2, Dickkopf-related protein 2; Hif-2, Hypoxia-inducible factor 2; TGF-β1, Transforming growth factor β1; VEGFα, Vascular endothelial growth factor α.