Table 1 Oligonucleotides used for real-time RT-PCR a
Gene |  | Sequence 5′-3′ | Gene ID |
|---|---|---|---|
RP29 | S | 5′-GGG TCA CCA GCA GCT CGA GA-3′ | NM_001032.3 |
AS | 5′-CAG ACA CGA CAA GAG CGA GA-3′ | ||
Leptin | S | 5′-GGC TTT GGC CCT ATC TTT TC-3′ | NM_000230.2 |
AS | 5′-GGA TAA GGT CAG GAT GGG GT-3′ | ||
DKK2 | S | 5′-GGG TTT TGC TGT GCT CGT-3′ | NM_014421.2 |
AS | 5′-TGG CTT TGG AGG AGT AGG TG-3′ | ||
Hif-1 | S | 5′-GAA AGC GCA AGT CCT CAA AG-3′ | NM_181054.2 |
AS | 5′-TGG GTA GGA CAT GGA GAT GC-3′ | ||
Hif-2 | S | 5′-TTG-ATG-TGG-AAA-CGG-ATG-AA-3′ | NM_001430.4 |
AS | 5′-GGA-ACC-TGC-TCT-TGC-TGT-TC-3′ | ||
Osteocalcin | S | 5′-CAT-GAG-AGC-CCT-CAC-A-3′ | NM_199173.4 |
AS | 5′-AGA-GCG-ACA-CCC-TAG-AC-3′ | ||
VEGFα | S | 5′-TGG-GCC-TTG-CTC-AGA-GCG-GA-3′ | NM_001171629.1 |
AS | 5′-GCC-TTG-CAA-CGC-GAG-TCTGT-3′ | ||
TGFβ1 | S | 5′-GCG-TGC-TAA-TGG-TGG-AAA-C-3′ | NM_000660.5 |
AS | 5′-GCT-GAG-GTA-TCG-CCA-GGA-A-3′ | ||
Sirt1 | S | 5′-GCT-GGA-ACA-GGT-TGC-GGG-AA-3′ | NM_012238.4 |
AS | 5′-GGG-CAC-CTA-GGA-CAT-CGA-GGA-3′ |