Figure 2

Development of glomerulonephritis and pneumonitis in the majority of scurfy mice, but not in wild-type mice . Scurfy (Sc) mice developed mesangial glomerulonephritis comparable to World Health Organization class II lupus nephritis in humans. Representative hematoxylin and eosin (H&E)–stained sections of a wild-type (WT) kidney (a) and a scurfy mouse kidney (b), a periodic acid-Schiff stain of a glomerulum from a scurfy mouse kidney (c). (d) Glomerulonephritis occurred in eight (89%) of nine scurfy mice, but not in controls (P = 0.0004). Lung involvement resembling lupus pneumonitis was found in all but one scurfy mouse (83%), but not in controls (P = 0.0152). Representative examples of direct immunofluorescence of a scurfy mouse kidney (e) and a WT kidney (f) are shown. Also shown are representative H&E-stained sections of a WT lung (g) and a scurfy lung (h), a higher-magnification image of a peribronchial inflammatory infiltrate in a scurfy lung (i) and the total lung inflammation score in scurfy and WT mice (j).