Figure 6

Cardiac and spleen mass. (A) Cardiac hypertrophy measurements in WT (n = 14) and ABIN1[D485N] mice (n = 13) cholesterol-fed mice: cardiac mass (mg) versus tibia length (mm) ± SEM (A). (B) Spleen mass (g ± SEM) in WT (n = 6) and ABIN1[D485N] (n = 7) cholesterol-fed animals. Unpaired Student t test. *P <0.05, ***P <0.001. ( C) Summary of follow-up measurements: High-density lipoproteins (HDLs) in WT-chow (n = 8), WT-cholesterol (n = 6), ABIN1[D485N]-chow (n = 9), and ABIN1[D485N]-cholesterol- fed mice (n = 9) and low-density lipoproteins and very-low-density lipoproteins (LDL/vLDL) in the same animals (mg/dl ± SEM). Plasma inflammatory markers at 4 weeks in WT-chow (n = 10), WT-cholesterol (n = 7), ABIN1[D485N]-chow (n = 10), and ABIN1[D485N]-cholesterol (n = 10)-fed animals for (A) IL-1α, (B) IL-6, and (C) IL-10 (pg/ml ± SEM). Microvascular responses at 4 weeks to endothelium-dependent acetylcholine in WT-chow (n = 11), WT-cholesterol (n = 11), ABIN1[D485N]-chow (n = 10) and ABIN1[D485N]-cholesterol (n = 10) fed mice and endothelium-independent sodium nitroprusside in the same mice (% change ± SEM). Cardiac hypertrophy measurements in WT (n = 14) and ABIN1[D485N] mice (n = 13) cholesterol-fed mice and spleen mass (gram ± SEM) in WT (n = 6) and ABIN1[D485N] (n = 7) cholesterol-fed animals. Results are expressed as group means ± SEM.