Figure 3
From: CYB5A polymorphism increases androgens and reduces risk of rheumatoid arthritis in women
CYB5A genotype affects pregnenolone metabolism in rheumatoid arthritis (RA) synovial fibroblasts. (A, B) Products of pregnenolone metabolism in 40 synovial fibroblast lines from RA patients genotyped for rs1790834 (n = 27, GG genotype; n = 13, genotypes AA/AG). (C, E) Product ratios indicate that the CYB5A A allele increases steroid 17,20-lyase activity of CYP17A1 gene product. Shown are the calculated ratios 17α-hydroxy-pregnenolone/7α-hydroxy- dehydroepiandrosterone (DHEA) (C), 17α-hydroxy-pregnenolone/17,20-lyase (D), and 17α-hydroxylase/17,20-lyase (E), where 17,20-lyase refers to the sum of all C19-steroids produced and 17α-hydroxylase refers to the sum of 17α-hydroxy-pregnenolone plus 17α-hydroxy-Prog. Significant differences in rs1790834 genotype-dependent metabolite levels or product ratios, respectively, were identified with the Mann-Whitney test: *P <0.05; **P <0.01. Δ5Adiol, Δ5-androstenediol; Adione, androstenedione; Preg, pregnenolone; Prog, progesterone; Testo, testosterone.