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Figure 5 | Arthritis Research & Therapy

Figure 5

From: High mobility group box 1 contributes to anti-neutrophil cytoplasmic antibody-induced neutrophils activation through receptor for advanced glycation end products (RAGE) and Toll-like receptor 4

Figure 5

Blockage of TLR4 and RAGE rather than TLR2 decreased ANCA antigens translocation, ANCA-induced respiratory burst and degranulation in HMGB1-primed neutrophils. Blockage of TLR4 and RAGE rather than TLR2 decreased expression of mPR3 on HMGB1-primed neutrophils (A) and concentration of MPO in the neutrophils culture supernatant (B). Blockage of TLR4 and RAGE rather than TLR2 decreased neutrophil respiratory burst induced by patient-derived PR3-ANCA-positive IgG (C) or MPO-ANCA-positive IgG (D) in HMGB1-primed cells. Blockage of TLR4 and RAGE rather than TLR2 decreased neutrophil degranulation induced by patient-derived PR3-ANCA-positive IgG (E) or MPO-ANCA-positive IgG (F) in HMGB1-primed cells. Bars represent mean ± standard deviation (SD) of repeated measurements on neutrophils of 8 independent experiments and donors. All statistical differences between groups were shown in the Table S2 in Additional file 3. ANCA, antineutrophil cytoplasmic antibody; HMGB1, high mobility group box-1; IgG, immunoglobulin G; MPO, myeloperoxidase; PR3, proteinase 3; RAGE, receptor for advanced glycation end products; TLR, Toll-like receptor.

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