Figure 7

MyD88 and NF-κ B inhibitors decreased ANCA antigens translocation, ANCA-induced respiratory burst and degranulation in HMGB1-primed neutrophils. Inhibition of MyD88 and NF-κB decreased expression of mPR3 on HMGB1-primed neutrophils (A) and concentration of MPO in the neutrophils culture supernatant (B). Inhibition of MyD88 and NF-κB decreased neutrophil respiratory burst induced by patient-derived PR3-ANCA-positive IgG (C) or MPO-ANCA-positive IgG (D) in HMGB1-primed cells. Inhibition of MyD88 and NF-κB decreased neutrophil degranulation induced by patient-derived PR3-ANCA-positive IgG (E) or MPO-ANCA-positive IgG (F) in HMGB1-primed cells. Bars represent mean ± standard deviation (SD) of repeated measurements on neutrophils of five independent experiments and donors. All statistical differences between groups were shown in the Table S3 in Additional file 3.