Fig. 4

Male DBA-2 mice had reduced numbers of CD3, CD22 and CD68 cell infiltration into bleomycin-challenged mouse skin. Representative images of a CD3, b CD22 and c CD68 immunohistochemical analysis sections of skin from male Balb/C, C57BL/6 and DBA/2 mice subjected to bleomycin (0.5 mg/ml) injections given every other day. d-f Relative number of CD3-, CD22- and CD68-positive cells in the dermis of male Balb/C, C57BL/6 and DBA/2mice in response to bleomycin injections. Original magnification ×400. All values represent means ± standard error of the mean (SEM); n = 6 in each group. Representative images of g CD3, h CD22 and i CD68 immunohistochemical analysis sections of skin from female Balb/C, C57BL/6 and DBA/2 mice subjected to bleomycin (0.5 mg/ml) injections given every other day. j-l Relative number of CD3-, CD22- and CD68-positive cells in the dermis of female Balb/C, C57BL/6 and DBA/2 mice in response to bleomycin injections. Original magnification ×400. All values represent means ± SEM; n = 6 in each group