Fig. 6

a Quantification of RA synovial tissue spontaneous secretion of MMP-3 (i), TIMP-3 (ii) and the MMP-3/TIMP-3 ratio (iii) following culture with OPN301 (1 μg/ml) or IgG control (1 μg/ml) (n = 4). b Decreased MMP-2 and MMP-9 expression in response to culture with OPN301 (1 μg/ml) compared to anti-IgG control antibody (1 μg/ml) in RA synovial culture supernatants as assessed by gelatin zymography (n = 3) (c). Representative photomicrograph demonstrating inhibition of RASFC migration in response to conditioned media from ex vivo RA synovial tissue treated with OPN301 (1 μg/ml) compared to IgG control (1 μg/ml) (n = 3) (d). Representative photomicrographs showing OPN301-conditioned media (1 μg/ml) inhibits RASFC invasion compared to IgG-conditioned media (i). Bar graph quantifying RASFC invasion (ii) (n = 4). Data is represented as mean ± SEM, ^* p <0.05, significantly different from control. IgG immunoglobulin G, MMP matrix metalloproteinase, RA rheumatoid arthritis, RASFC rheumatoid arthritis synovial fibroblast cells, TIMP tissue inhibitor of metalloproteinase