Fig. 2

Dose-response curves representing the percentage of ACPA targeted by fibrinogen α chain peptides. ACPA was incubated with the respective peptides at different concentrations for one hour at room temperature, before proceeding with the anti-CCP2 ELISA. a. ACPA (pool I) blocking with citrulline or arginine-containing fibrinogen peptides from the α chain, 573 (left panel) and 591 (right panel). b. ACPA (pool II) blocking with citrulline or arginine-containing fibrinogen peptides from the α chain, 573 (left panel) and 591 (right panel). X-axes show peptide amount (nmol). Y-axes show ACPA inhibition levels (%). Circles represent means of two to seven experiments per ACPA pool and error bars represent SEM. ^* P <0.05, ^** P <0.001