Fig. 3

Impaired expression of co-stimulatory molecules and secretion of lower amounts of cytokine involved in T-cell activation by pre-naïve B cells. a Mean fluorescence intensity (MFI) of CD80, CD86, CD54, and HLA-DR expression of naïve, pre-naïve, and memory B-cell population on days 0–2 after stimulation with CD154-L cells (n = 18, cumulative data from 10 independent experiments). Data are expressed as mean MFI ± standard error (SE). b MFI of CD80 and CD86 expression of each B-cell population on day 1 of cultures described in (a) in medium alone or in the presence of anti-IL-10+ anti-IL-10Ra, rhIL-10, and rhIL-10+ anti-IL-10+ anti-IL-10Ra (n = 18, cumulative data from 10 independent experiments). Data are expressed as mean ± SE fold change over non-treated samples. c IL-6 and TNF-α levels in culture supernatants on day 1 of culture as described in (b). IL-6 and TNF-α secretion in the culture supernatants was measured by multiplex assay (n = 18, cumulative data from 10 independent experiments). Data are expressed as mean ± SE. *P < 0.05, **P < 0.01. ***P <0.001. IL-10 interleukin-10, TNF-α tumor necrosis factor-alpha, rhIL-10 recombinant IL-10, anti-IL-10Ra anti-IL-10 receptor antibody