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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Recombinant human SIRT1 protects against nutrient deprivation-induced mitochondrial apoptosis through autophagy induction in human intervertebral disc nucleus pulposus cells

Fig. 4

Effect of recombinant human silent mating type information regulator 2 homolog 1 (rhSIRT1) on apoptosis of human nucleus pulposus cells. a Flow cytometric analysis of annexin V conjugated with fluorescein isothiocyanate (Annexin V-FITC) and propidium iodide–stained nucleus pulposus cells from intervertebral discs (IVDs) of Pfirrmann grades II and IV after 3, 7, and 14 days of treatment with 10 μM rhSIRT1 or 5 mM 3-methyladenine (3-MA) under normal nutrition (N) conditions with 10 % (v/v) fetal bovine serum (FBS) or low nutrition (LN) conditions with 1 % (v/v) FBS. b Changes in the percentage of apoptotic NP cells from IVDs of Pfirrmann grades II and IV after 3, 7, and 14 days of treatment. Data are expressed as the mean ± standard deviation (n = 5 for cells from grade II IVDs and n = 8 for cells from grade IV IVDs). *P < 0.05; † P < 0.01, three-way analysis of variance with the Tukey–Kramer post hoc test

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Arthritis Research & Therapy

ISSN: 1478-6362

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