Table 1 RT-PCR and qRT-PCR primers
From: Histone deacetylase inhibition activates Nrf2 and protects against osteoarthritis
Genes | RT-PCR primers |
|---|---|
MMP-1 | Forward: GTCAGGGGAGATCATCGG |
MMP-1 | Reverse: GCCCAGTACTTATTCCCT |
MMP-3 | Forward: ATGCCCACTTTGATGATGATGAAC |
MMP-3 | Reverse: CCACGCCTGAAGGAAGAGATG |
MMP-13 | Forward: GCTTAGAGGTGACTGGCAA |
MMP-13 | Reverse: CCGGTGTAGGTGTAGATAGGAA |
GAPDH | Forward: CTGCCGTCTAGAAAAACC |
GAPDH | Reverse: CCAAATTCGTTGTCATACC |
Nrf2GT | Forward: TGGACGGGACTATTGAAGGCTG |
Nrf2-WT | Reverse: CGCCTTTTCAGTAGATGGAGG |
Nrf2-KO | Reverse: GCGGATTGACCGTAATGGGATAGG |
Genes | qRT-PCR primers |
IL-1β | Forward: ATGGCAGAAGTACCTAAGCTCGC |
IL-1β | Reverse: ACACAAATTGCATGGTGAAGTCAGTT |
TNF-α | Forward: ATGAGCACAGAAAGCATGATCCGC |
TNF-α | Reverse: CCAAAGTAGACCTGCCCGGACTC |
IL-6 | Forward: ACACACTGGTTCTGAGGGAC |
IL-6 | Reverse: TACCACAAGGTTGGCAGGTG |
MMP-1 | Forward: GCCACAAAGTTGATGCAGTT |
MMP-1 | Reverse: GCAGTTGAACCAGCTATTAG |
MMP-3 | Forward: ATGAAAATGAAGGGTCTTCCGG |
MMP-3 | Reverse: GCAGAAGCTCCATACCAGCA |
MMP-13 | Forward: ATGCATTCAGCTATCCTGGCCA |
MMP-13 | Reverse: AAGATTGCATTTCTCGGAGCCTG |
NQO1 | Forward: TTTAGGGTCGTCTTGGCA |
NQO1 | Reverse: GTCTTCTCTGAATGGGCCAG |
HO-1 | Forward: ACATCGACAGCCCCACCAAGTTCAA |
HO-1 | Reverse: CTGACGAAGTGACGCCATCTGTGAG |
β-actin | Forward: TGACGGGGTCACCCACACTGTGCCCATCTA |
β-actin | Reverse: CTAGAAGCATTTGCGGTGGACGATGGAGGG |