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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Enhanced activity of NLRP3 inflammasome in peripheral blood cells of patients with active rheumatoid arthritis

Fig. 5

Assessment of the effect of inhibition of various caspases in IL-1β maturation upon nucleotide binding domain and leucine-rich repeat pyrin 3 domain (NLRP3) activation. Whole blood cells from patients with rheumatoid arthritis (RA) (n = 20) were primed with Toll-like receptor (TLR)4 (lipopolysaccharide (LPS), 250 pg/mL, 2 h) (a), TLR3 (poly IC, 50 μg/mL, 2 h) (b) or TLR2 (pam3CysSK 200 ng/mL, 2 h) (c) ligands and sequentially pulsed with ATP (ATP 5 mM, 20 min). Inhibitors of caspase-1 (a) or caspase-3/7 (b) or caspase-8 (c) were applied. IL-1β secretion in cell supernatants was quantified by ELISA (pg/mL). pIC polyinosinic-polycytidylic acid, Pam pam3CysSK, Casp1Inh caspase1 inhibitor, Casp3/7Inh caspase3 and 7 inhibitor, Casp8Inh caspase8 inhibitor

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