Fig. 2

The characteristics of survivin expression in systemic sclerosis (SSc) dermal lesions. a Arrows indicate survivin-positive cells (stained by red). (b-d) The multiple immunofluorescence method for SSc and non-SSc dermal lesions with anti-survivin, anti-CD1a, anti-CD4 and anti-CD69 antibodies was performed. b Arrows indicate CD1a⁺ survivin⁺ cells. c Staining with anti-CD1a, anti-CD69 antibodies. d Staining with anti-CD4 and anti-CD69 antibodies. Arrows indicate CD69⁺ CD4⁺ cells. e A Western blot analysis revealed that the anti-survivin antibody reacted to all three variants. On the other hand, anti-survivin-ΔEx3 and anti-survivin-2B antibodies reacted with each respective variant only. f Immunohistochemistry (IHC) using anti-survivin-ΔEx3 and -2B antibodies (stained by red). Right figures show positive controls on the same slide