Fig. 4

The role of survivin in CD1a⁺ monocyte-derived dendritic cells (Mo-DCs). a CD14⁺ monocytes change into CD1a⁺CD14⁻ DCs by culturing with granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4. b A Western blot analysis showed that BIRC5 mRNA expressed the survivin-WT protein in Mo-DCs. c Mo-DCs were transfected with survivin-wild type (WT) and mock at day 0, and were subsequently stimulated with GM-CSF and IL-4. At 25 and 50 hours, the samples were analyzed using fluorescence-activated cell sorting (FACS) analyses. The percentages in the figures indicate the ratio of CD1a⁺CD14⁻ cells. d-f Mo-DCs were transfected with survivin-WT and mock at day 6, and the following FACS analyses were performed at 24 hours. d Harvested Mo-DCs were stained with FVD520 and anti-Ki-67 antibodies. The positive rate was calculated as the number of Ki-67-positive cells among FVD520-negative (living) cells. Data are presented as the percentage of control cells. Mean ± SEM. n = 4 (both groups). ^* t test, p < 0.05. e, f Harvested Mo-DCs were stained with 7-amino-actinomycinD (7-AAD). The percentages of G2/M phase cells (e) and apoptotic cells (f) were calculated. Data are presented as the percentage of control cells. Mean ± SEM, n = 5 (both groups). t test, ^* p < 0.05, ^** p < 0.01