Fig. 9

Anti-CD68 antibody (ED1) immunoreactivity in lumbar dorsal root ganglia (DRGs) and its correlations with growth-associated protein 43 (GAP-43)–positive intraepidermal nerve fibres (IENFs) and behavioural data. Plot of the time course of GAP-43–positive IENFs in the left paw pad skin and ED1 immunoreactivity (macrophage invasion) in the lumbar DRGs (a). Correlation between ED1-immunoreactive cells in the DRGs and weight-bearing. Relative weight bearing for control animals was defined as 50 %, with equal weight on both legs. Control values are parenthesized (b). Correlation between ED1-immunoreactive cells in the DRGs and primary hyperalgesia. Control values in parentheses were set at 234.5 g, which was the average baseline value from animals before induction of AIA (SD was ±20.6 g) (c). Relationship of ED1 immunoreactivity and secondary hyperalgesia in the paw. Control values in parentheses were determined as the mean of baseline values from acute and chronic AIA animals before induction of AIA (27.9 g ± 7.6 g) (d)