Fig. 6From: Citral alleviates an accelerated and severe lupus nephritis model by inhibiting the activation signal of NLRP3 inflammasome and enhancing Nrf2 activationIn vitro effect of Citral on NLRP3 inflammasome activation in J774A.1 macrophages. a IL-1β secretion by lipolysachharide (LPS) + ATP-activated macrophages. b Western blots showing caspase-1 activation in LPS + ATP-activated macrophages. c ATP-induced IL-1β secretion by LPS-primed macrophages. d ATP-induced caspase-1 activation in LPS-primed macrophages. e Representative western blots showing the effect of treatment with different concentrations of Citral for 30 minutes before addition of LPS for 6 h on NLRP3 and pro-IL-1β levels in J774A.1 macrophages. f and g Semiquantitative analysis of the NLRP3/β-actin ratio (f) and pro-IL-1β/β-actin ratio (g). The data are expressed as the mean ± SD for three separate experiments; the histogram shows the quantification expressed as the mean ± SD; *p <0.05, **p <0.01, ns not significantBack to article page