Fig. 3

Therapeutic comparison of Janus kinase (JAK) + spleen tyrosine kinase (SYK) inhibition and anti-TNF therapy in glucose-6-phosphate isomerase (G6PI)-induced arthritis. DBA/1 mice were immunized with G6PI + complete Freund’s adjuvant on day 0, after T regulatory cell depletion with anti-CD25 Ab (days −8 and −11). Treatments were administered starting on day 12 (red dotted line). Samples were obtained on day 36. a Time course for arthritis scores. b Mean weight of both inguinal lymph nodes. c Mean ankle thickness of both hind paws measured with a manual caliper. d Histopathological scores for tarsus and pannus formation in both phalanges and tarsus. Representative histological sections of tarsal joints are also shown (bar 500 μM). e Time course for arthritis scores for mice with mild, moderate and severe arthritis at the time of treatment initiation. Graphs show mean (± standard error of the mean) for 2–10 mice/group, *p <0.05 versus vehicle control. N naive, Veh vehicle