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Fig. 7 | Arthritis Research & Therapy

Fig. 7

From: Simultaneous inhibition of JAK and SYK kinases ameliorates chronic and destructive arthritis in mice

Fig. 7

Effects of Janus kinase (JAK) + spleen tyrosine kinase (SYK) inhibition on effector and memory T and B cells. a Proliferation of purified T cells stimulated with 1 μg/ml anti-CD3 and 0.5 μg/ml anti-CD28 Abs in the presence of kinase inhibitors (0.5 μM) for 72 h. b Cytokine secretion by splenocytes from arthritic mice in vitro re-exposed to 20 μg/ml recombinant human glucose-6-phosphate isomerase (G6PI) and treated with JAK inhibitor (JAKi) + SYK inhibitor (SYKi) (0.5 μM) for 72 h. c-f BALB/c mice were immunized and challenged with ovalbumin (OVA) + alum; 2 weeks after challenge, mice were treated with kinase inhibitors for 11 days. c-e Numbers of memory CD4+ T cells (CD3+CD4+CD44hi), antigen-specific (CD3+CD4+CD44hiCD40L+) and cytokine-producing (CD40L+cytokine+) memory CD4+ T cells upon OVA re-exposure in vitro. f Numbers of OVA-specific memory plasma cells (CD138+κλhiOVA+ 5-Bromo-2′-deoxyuridine+). Graphs show mean (+ standard error of the mean) for 3–5 mice/group, *p <0.05 versus media or vehicle control. Med media, Veh vehicle

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