Table 1 List of primers used for the detection of DKK-1, BMP-2, BMPRII and LRP-5
Target | Sequences |
|---|---|
Mouse DKK-1 | Forward 5’-GAG GGG AAA TTG AGG AAA GC-3’ |
Reverse 5’-GCA GGT GTG GAG CCT AGA AG-3’ | |
Mouse BMP-2, | Forward 5’-GGG ACC CGC TGT CTT CTA GT-3’ |
Reverse 5’-TCA ACT CAA ATT CGC TGA GGA C-3’ | |
Mouse BMPRII | Forward 5’-TTG GGA TAG GTG AGA GTC GAA T-3’ |
Reverse 5’-TGT TTC ACA AGA TTG ATG TCC CC-3’ | |
Mouse LRP-5 | Forward 5’-CAG GTG CTT GTG TGG AGA GA-3’ |
Reverse 5’-GTC CAT GAC GAA GTC CAG GT-3’ | |
Mouse GAPDH | Forward 5’-AGC CAC ATC GCTCAG ACA-3’ |
Reverse 5’-GCC CAA TAC GAC CAAATC C-3’ | |
Human DKK-1 | Forward 5’-CAT CAG ACT GTG CCT CAG GA-3’ |
Reverse 5’-CCA CAG TAA CAA CGC TGG AA-3’ | |
Human GAPDH | Forward 5’-CGT CTT CAC CAC CAT GGA GA-3’ |
Reverse 5’-CGG CCA TCA CGC CAC AGT TT-3’ |