Table 2 Primer sequences for the genes measured in this experiment
From: Hedgehog signalling does not stimulate cartilage catabolism and is inhibited by Interleukin-1β
Gene | Species | Sequence (5′-3′) |
|---|---|---|
Patched 1 | Bovine | F-ATGTCTCGCACATCAACTGG |
R-TCGTGGTAAAGGAAAGCACC | ||
Gli1 | Bovine | F-ACCCCACCACCAGTCAGTAG |
R-TGTCCGACAGAGGTGAGATG | ||
ADAMTS-5 | Bovine | F- GCCCTGCCCAGCTAACGGTA |
R- CCCCCGGACACACACGGAA | ||
MMP-13 | Bovine | F- CCCTTGATGCCATAACCAGT |
R- GCCCAAAATTTTCTGCCTCT | ||
18S rRNA | Bovine | F- GCAATTATTCCCCATGAACG |
R- GGCCTCACTAAACCATCCAA | ||
ADAMTS-5 | Human | F- CCTTGTGGAAAGGGGAGAAT |
R- ACAGTGACGATAGGCAAACT | ||
MMP-13 | Human | F- AGCCACTTTATGCTTCCTGA |
R- TCAAACTGTATGGGTCCGTT | ||
18S rRNA | Human | F- CGGCTACCACATCCAAGGAA |
R- AGCTGGAATTACCGCGGC |