Fig. 3

Retention of Cy5.5-1-11E scFv in mice following i.a. injection 8 week post-DMM surgery. a Representative fluorescent overlay images of mice 8 weeks post-surgery and after 120 and 336 hours i.a injection of 1 μg Cy5.5-1-11E-scFv or Cy5.5-C7-scFv. Green circles denote the regions of interest used for quantification; green arrow indicates the right DMM-operated knee (RK); LK is the non-operated contralateral knee. The amount of injected Cy5.5-1-11E-scFv (b) and Cy5.5-C7-scFv (c) remaining in the knee joints are expressed as a percentage with 100 % taken as the amount present immediately after i.a. (time 0) injection. Data are mean ± SEM, n = 7 for 1-11E-injected group and n = 5 for C7-injected group. *p < 0.05 between the half life of 1-11E-scFv in the DMM vs the other groups. DMM destabilised medial meniscus-operated knee, DMM Contra non-operated contralateral knee, scFv single chain fragment variable, Sham sham-operated RK, Sham Contra non-operated contralateral knee