Fig. 4

Localization of Cy5.5-1-11E-mAb in mice following i.v injection 4 weeks post-DMM surgery. a Representative fluorescent overlay images of mice injected i.v. with 10 μg Cy5.5-1-11E-mAb or Cy5.5-C7-mAb 4 weeks post-surgery. Green circles denote the regions of interest used for quantification; green arrow indicates the right DMM operated knee (RK); LK is the non-operated contralateral knee. b, c Quantification of the amount of antibody detected in the knee joints at the times indicated. Data are mean ± SEM, n = 8. *p < 0.1 by two-way analysis of variance between DMM and sham groups. DMM destabilised medial meniscus-operated knee, DMM Contra non-operated contralateral knee, scFv single chain fragment variable, Sham sham-operated RK, Sham Contra non-operated contralateral knee