Skip to main content
Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: High-mobility group box 1 potentiates antineutrophil cytoplasmic antibody-inducing neutrophil extracellular traps formation

Fig. 5

NETs formation was dependent on NADPH oxidase in the presence of HMGB1 and ANCA-positive IgG. Blockage of NADPH oxidase decreased DNA release by MPO-ANCA-positive IgG (a) or PR3-ANCA-positive IgG (b) in HMGB1-pretreated cells. Blockage of NADPH oxidase decreased percentage of NETs formation by MPO-ANCA-positive IgG (c) or PR3-ANCA-positive IgG (d) in HMGB1-pretreated cells. Bars represent mean ± SD of repeated measurements on neutrophils of 13–14 independent experiments and 9 donors. ANCA antineutrophil cytoplasmic antibody, DPI diphenyleneiodonium, HMGB1 high-mobility group box 1, IgG immunoglobulin G, MPO myeloperoxidase, NETs neutrophil extracellular traps, PR3 proteinase 3

Back to article page

Arthritis Research & Therapy

ISSN: 1478-6362

Contact us