Fig. 2

Decreased CFA-evoked neutrophil myeloperoxidase activity and vascular leakage in the ankle joints of TRPA1 KO mice. a Representative images of luminol activity showing neutrophil myeloperoxidase activity, and (b) quantification of luminescence in the diseased joints on days 2 and 7. c Representative images of indocyanine green fluorescence demonstrating plasma protein extravasation in the ipsilateral joints, and (d) quantitative analysis of the fluorescence intensity at the same time points. Data are shown as means ± SEM of n = 5–7 mice/group, *p < 0.05, ***p < 0.001 (vs. WT ipsilateral joint), ^# p < 0.05, ^### p < 0.001 (vs. respective contralateral joint); two-way ANOVA followed by Bonferroni’s multiple comparison test. ANOVA analysis of variance, CFA complete Freund’s adjuvant, KO knockout, SEM standard error of the mean, TRPA1 transient receptor potential akyrin 1, WT wildtype