Fig. 3

Decreased severity of CFA-induced histopathological alterations in the tibiotarsal joint of TRPA1 KO mice. Representative slides stained with hematoxylin and eosin of (a) an intact tibiotarsal joint of a WT, (c) arthritic WT, and (d) arthritic TRPA1 KO mouse obtained on day 10 (40× magnification; mononuclear cell infiltration (mci), synovial cell lining hyperplasia (sh)). b Semiquantitative composite arthritis scores obtained on the basis of synovial cell lining hyperplasia, mononuclear cell infiltration and cartilage destruction. Box plots represent medians of composite scores for n = 4–5 mice/group; ***p < 0.001 (vs. WT ipsilateral joint), ^### p < 0.001 (vs. respective contralateral joint); one-way ANOVA followed by Bonferroni’s multiple comparison test. ANOVA analysis of variance, CFA complete Freund’s adjuvant, KO knockout, TRPA1 transient receptor potential akyrin 1, WT wildtype