Fig. 5

Similar histopathological severity of the MIA-injected knees of TRPA1 KO and WT mice. Representative slides stained with Safranin O of (a) an intact knee joint, (c) osteoarthritic WT, and (d) TRPA1 KO mouse knee obtained on day 22 (200× magnification; roughened cartilage surface (cs), moderate disorganization and cell loss (cl), reduced matrix staining (ms), disrupted tidemark integrity (ti)). b Modified Mankin semiquantitative scores obtained on the basis of structure, cellularity, matrix staining, tidemark integrity, synovial hyperplasia, synovial inflammatory cells infiltration, and osteophyte formation. Box plots represent medians of composite scores for n = 6–9 mice/group; ^### p < 0.001 (vs. respective contralateral joint), one-way ANOVA followed by Bonferroni’s multiple comparison test. ANOVA analysis of variance, KO knockout, MIA monosodium iodoacetate, TRPA1 transient receptor potential akyrin 1, WT wildtype