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Table 5 Histological analysis in the MMT/MCLT studies (fluocinolone)

From: Randomized controlled studies on the efficacy of antiarthritic agents in inhibiting cartilage degeneration and pain associated with progression of osteoarthritis in the rat

Animal Knee Medial tibial cartilage degeneration scorea Tibial cartilage degeneration width Depth ratio, any matrix changeb Medial tibial osteophytes Medial femoral cartilage degeneration scorea Bone score Total joint score without femur Total joint score
   Three-zone total Zone 1 (Outside) Zone 2 (Middle) Zone 3 (Inside) Totalc (μm) Sigd (μm) Mean Zone 2 Scoree Measure (μm)     
1 R 6.33 4.33 2.00 0.00 1133.33 633.33 0.34 0.05 3.00 413.33 2.00 4.00 9.33 11.33
2 R 4.67 3.33 1.33 0.00 1000.00 433.33 0.28 0.06 1.33 280.00 3.00 1.00 6.00 9.00
3 R 7.00 4.67 2.33 0.00 1133.33 766.67 0.39 0.16 3.00 426.67 2.33 2.00 10.00 12.33
4 R 7.67 4.67 2.67 0.33 1600.00 800.00 0.42 0.31 4.67 586.67 3.00 3.00 12.33 15.33
5 R 5.33 4.00 1.33 0.00 1266.67 566.67 0.33 0.05 3.00 400.00 1.33 3.00 8.33 9.67
6 R 6.00 4.00 2.00 0.00 1100.00 666.67 0.35 0.10 2.67 380.00 2.00 3.00 8.67 10.67
7 R 4.67 3.00 1.67 0.00 1400.00 466.67 0.29 0.13 2.00 333.33 1.00 3.00 6.67 7.67
8 R 5.00 3.00 2.00 0.00 1066.67 433.33 0.32 0.03 2.33 333.33 1.00 2.00 7.33 8.33
Mean   5.83 3.88 1.92 0.04 1212.50 595.83 0.34 0.11 2.75 394.17 1.96 2.63 8.58 10.54
SE 0.39 0.24 0.16 0.04 70.69 51.35 0.02 0.03 0.34 32.50 0.28 0.32 0.71 0.88
t test to G1 0.19 0.23 0.23 1.00 0.27 0.05 0.46 0.18 0.02 0.01 0.77 0.11 0.04 0.12
Percentage 0.11 0.10 0.13 0.00 0.08 0.18 0.06 0.41 0.30 0.23 −0.07 0.19 0.18 0.14
  1. SE, standard error; Sig, significant; G1, group 1
  2. Shown are results of the analysis of toluidine-stained sections from three levels within the joints of a group of eight animals treated systemically with 10 μg/kg fluocinolone for 21 days. Note that the cartilage degeneration and total joint scores depicted in Table 4 were not statistically different compared with the control group treated with saline (not shown). Several other measures were significantly different (t test results in boldface type). To view similar data for all the groups in both models, see Additional file 1
  3. aCartilage degeneration score = depth (0–5) for each of three zones, then summed (mean of three-step section)
  4. bMean lesion depth in micrometers versus depth to tidemark in center of zone in the tibial plateau (mean of three-step section)
  5. cWidth of any cartilage lesion (mean of three-step section)
  6. dWidth of cartilage degeneration extending >50 % of total thickness (mean of three-step section)
  7. eOsteophyte scores 1 = small up to 299 μm, 2 = medium 300–399 μm, 3 = large 400–499 μm, 4 = very large 500–599 μm, 5 = very large >600 μm